Table 1 Multiple reaction monitoring (MRM) transition table with parameters

	Parent m/z	Product m/z	Dwell time (ms)	Q1 pre bias (V)	CE	Q3 pre bias (V)
GSH	308	179	65	−11	−19	−23
GSSG	613	355	65.6	−14	−20	−25
GSH-NEM	433	304	65.6	−10	−17	−21
	433	201	65.6	−16	−21	−21
GSH-d5-NEM	438	309	65.6	−10	−17	−21
	438	206	65.6	−16	−21	−21
IS	441	312	100	−10	−17	−21
	441	206	100	−16	−21	−21
sLG	380	223.05	100	−11	−18	−24
	380	148.1	100	−11	−22	−30
	380	76.05	100	−11	−37	−14
CysGly-NEM	304	287	65.6	−11	−14	−19
	304	201	65.6	−11	−15	−21
CysGly-d5-NEM	309	292	65.6	−11	−14	−19
	309	206	65.6	−11	−15	−21
Cys-NEM	247.11	230.05	65.6	−17	−14	−24
	247.11	201.05	65.6	−14	−13	−21
Cys-d5-NEM	252.11	235.05	65.6	−17	−14	−24
	252.11	206.05	65.6	−14	−13	−21
GluCys-NEM	376	247	65.6	−14	−15	−35
	376	201.1	65.6	−14	−20	−20
GluCys-d5-NEM	381	252.05	65.6	−14	−15	−25
	381	206.1	65.6	−14	−20	−20
HCys-NEM	261	56.05	65.6	−13	−16	−22
	261	215.05	65.6	−13	−12	−23
HCys-d5-NEM	266	56.05	65.6	−13	−16	−22
	266	222.05	65.6	−13	−12	−23

CE collision energy, Q-quadrupole (GSH glutathione, GSSG glutathione disulfide, NEM N-ethylmaleimide, IS internal standard (¹³C¹⁴,N-GSH-d5-NEM), sLG S-lactoylglutathione, CysGly cysteinyl glycine, GluCys gamma glutamyl-cysteine, Cys cysteine, HCys homocysteine).

Quick links

Search