Fig. 1: Assignment of the BiP NBD ADP·Pi-bound state.
From: Mechanism of ATP hydrolysis in the Hsp70 BiP nucleotide-binding domain
a 2D [13C,1H]-methyl-TROSY spectra of methyl-labeled BiP in the presence of 5 mM ADP·Pi. Sequence-specific resonance assignments are indicated. b Graphical representation of the NOE network around methionine 263 (pink). Each arrow tip corresponds to one observed NOE cross peak between two methyl groups. c Completeness of the BiP Ile, Met and Val methyl NOE network, as expected from the structure PDB 5EVZ. For each distance range of the interproton distance, the numbers of observed/expected NOEs are indicated, and the percentage given as a grey bar. d Location of the assigned methyl groups (green spheres) in a 3D structure (PDB 5EVZ).
