Fig. 2: Reaction scheme and NMR fingerprints for the BiP NBD functional cycle. | Nature Communications

Fig. 2: Reaction scheme and NMR fingerprints for the BiP NBD functional cycle.

From: Mechanism of ATP hydrolysis in the Hsp70 BiP nucleotide-binding domain

Fig. 2: Reaction scheme and NMR fingerprints for the BiP NBD functional cycle.

a Scheme of the BiP NBD functional cycle including its kinetic parameters. See theory section for details. b Sections of 2D [13C,1H]-methyl-TROSY spectra of residues Val50cγ1, I52cδ1 and V172cγ2 for the equilibrium experiments of NBD apo (grey), NBD with 5 mM Pi (green), NBD with 5 mM ADP (light-blue) and NBD with 5 mM ADP·Pi (dark-blue).

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