Fig. 5: Second-layer septin filaments are oriented matching first-layer filaments on yeast polar extract SLBs.
From: Septin higher-order structure on yeast membranes in vitro
a Sequential frames during a HS-AFM force-sweep experiment to remove second-layer septin filaments. b Magnified views of timepoints t = 0 s (covered with second-layer filaments) and t = 130 s (first-layer filaments). c Contrast enhanced images of (b) showing matching orientation of the second-layer and first-layer filaments. Symbols * and ** indicate two regions (dashed outlines) with different yet inter-layer matching filament orientation. Experiment is representative of 27 HS-AFM movies across 7 biological replicates.
