Fig. 3: Single-cell RNA-sequencing of proximal small bowel crypts and villi reveal an expansion of an Lgr5(−) intestinal stem/progenitor population that is reversed after UCB Ab7326 treatment. | Nature Communications

Fig. 3: Single-cell RNA-sequencing of proximal small bowel crypts and villi reveal an expansion of an Lgr5(−) intestinal stem/progenitor population that is reversed after UCB Ab7326 treatment.

From: Epithelial GREMLIN1 disrupts intestinal epithelial-mesenchymal crosstalk to induce a wnt-dependent ectopic stem cell niche through stromal remodelling

Fig. 3: Single-cell RNA-sequencing of proximal small bowel crypts and villi reveal an expansion of an Lgr5(−) intestinal stem/progenitor population that is reversed after UCB Ab7326 treatment.The alternative text for this image may have been generated using AI.

A T-SNE plot of Epcam-expressing epithelial cells in the proximal small bowel of wild-type, Vil1-Grem1, and UCB Ab7326-treated Vil1-Grem1 mice, classified by cell type, with accompanying subplots separated by mouse genotype/treatment and tissue compartment B The number of cells of each epithelial cell type, as well as the corresponding percentage of total epithelial cells of each cell type for each sample. The number of stem progenitor cells in either the crypt or villi/polyps show a substantial expansion of the stem progenitor cell population in both the crypt and villi of the Vil1-Grem1 mice, which is reversed after subjecting the Vil1-Grem1 mice to 10 weeks of treatment with UCB Ab7326. C The expanded stem progenitor cell population in the Vil1;Grem1 mice is characterised by increased expression of Mki67, Sox9, Olfm4 and Fgfbp1.

Back to article page