Fig. 1: m6A methylome on coding and noncoding RNAs in the mFC regions of human brains.

a Piecharts showing the m6A peak distribution based on genomic locations in post-mortem human mFC tissues from Normal and AD donors, respectively. b A model illustrating major categories of de novo identified transcripts, including pre-mRNAs and ncRNAs. c A piechart showing the numbers of transcripts identified by the de novo calling. d A barplot showing the assignment of m6A peaks to various de novo called transcripts. e Genomic tracks showing an example of ncRNA (a promoter-antisense RNA from the SOX1 locus) having m6A peaks in human Normal and AD brains. Input and m6A represent the average signals of RNA-seq and MeRIP-seq datasets in this work, respectively. The arrows indicate m6A peaks. f Similar to E, example tracks showing intronic L1 elements overlap m6A peaks in the intron of GPHN gene. (+) and (-) indicate the Watson and Crick strands, respectively. g A volcano plot showing differential m6A peaks between Normal and AD brains. Red and blue dots represent hyper- and hypo-methylated m6A sites in AD. P values were calculated by a two-tailed student’s t-test. h Metaplots showing the aggregated m6A ratios of hyper- and hypo-methylated m6A sites.