Fig. 6: RBM15 regulates SWI/SNF complex assembly in mESCs.

a Schematic of the m⁶A RNA methylation pathway that regulates mRNA stability and translation. Created in BioRender https://BioRender.com/houenb9. b Western blot analysis of SWI/SNF subunits in RBM15 KO and NT mESCs. (n = 3). Note: B-ACTIN is a subunit of the SWI/SNF complex. c Graph shows quantification of replicates normalized to total protein stain, depicted as mean ± SEM (n = 3). Significance calculated using two-sided t-test. d Graph shows relative abundance of SWI/SNF subunits in complexes immunoprecipitated from RBM15 KO and NT mESCs. SWI/SNF complexes were isolated via V5-tagged SS18 subunits and peptide abundance was determined by MS1 based label-free quantitative immunoprecipitation mass-spectrometry (p-val <0.05, n = 3). P-values were calculated using ANOVA test. e Graph shows enrichment of SWI/SNF subunits relative to the ATPase subunit SMARCA4 following V5-SWI/SNF IP-MS. Fold change calculated over SMARCA4 depicted as mean ± SEM (n = 3). f Schematic of SWI/SNF complexes isolated from RBM15 KO mESCs, core subunits of the complex are enriched relative to the ATPase/ARP subunits leading to impaired SWI/SNF activity. Created in BioRender https://BioRender.com/887opbj. g Heatmap shows dynamic changes in m⁶A levels on 96/379 chromatin regulator genes profiled in m⁶A-RIP-seq analysis of RBM15 KO mESCs. Source data are provided as a Source Data file.