Fig. 4: Cotranscriptional folding of the C. beijerinckii pfl ZTP riboswitch expression platform.
From: Sequential structure probing of cotranscriptional RNA folding intermediates
a, b Secondary structures of the C. beijerinckii pfl riboswitch expression platform folding intermediates that were assessed by TECprobe-LM in the absence (a) and presence (b) of 1 mM ZMP colored by reactivity. c Transcript length distribution for the pre-wash, pre-chase, and post-chase samples. Traces are the average of n = 2 replicates. d Difference in reactivity (1 mM ZMP − 0 mM ZMP) observed for the pre-wash, pre-chase, and post-chase samples. Differences were calculated from the average reactivity values shown in (e) and (f). e, f Comparison of reactivity profiles of pre-wash and pre-chase samples (upper plot) and of pre-chase and post-chase samples (lower plot) in the absence (e) and presence (f) of 1 mM ZMP. Solid lines are the average of n = 2 replicates and reactivity values for individual replicates are shown as points. Source data are provided as a Source Data file. RNAP, RNA polymerase; PK, pseudoknot; SAv, streptavidin; BzCN, benzoyl cyanide.
