Fig. 6: Cotranscriptional folding of the B. cereus crcB fluoride riboswitch expression platform.
From: Sequential structure probing of cotranscriptional RNA folding intermediates
a, b Secondary structures of the B. cereus crcB fluoride riboswitch expression platform folding intermediates that were assessed by TECprobe-LM in the absence (a) and presence (b) of 10 mM NaF colored by reactivity. Sequence within the RNAP footprint is not shown. c Transcript length distribution for the pre-wash, pre-chase, and post-chase samples. Traces are the average of n = 2 replicates. d Difference in reactivity (10 mM NaF − 0 mM NaF) observed for the pre-wash, pre-chase, and post-chase samples. Differences were calculated from the average reactivity values shown in (e) and (f). e, f Comparison of reactivity profiles of pre-wash and pre-chase samples (upper plot) and of pre-chase and post-chase samples (lower plot) in the absence (e) and presence (f) of 10 mM NaF. Solid lines are the average of n = 2 replicates and reactivity values for individual replicates are shown as points. Source data are provided as a Source Data file. RNAP, RNA polymerase; PK, pseudoknot; SAv, streptavidin; BzCN, benzoyl cyanide.
