Fig. 4: The tumor (tumor cells) targeting and antitumor efficacy of MCNs/Ln/GD/FR NPs against subcutaneous melanoma.
From: Synthetic carbon-based lanthanide upconversion nanoparticles for enhanced photothermal therapy
A The expression of folate receptors in OCM-1 and ARPE-19 through Western Blot (n = 3 independent experiments). B one scheme of the expression of folate receptors in OCM-1 and ARPE-19. C The confocal microscope luminescent imaging of ARPE-19 and OCM-1 cells co-cultured with 200 μg mL-1 MCNs/Ln/D/FR NPs for 6 h. D Fluorescence quantitative analysis diagram of cells co-cultured with 200 μg mL−1 MCNs/Ln/D/FR NPs. E A simple scheme illustrating the procedures of subcutaneous melanoma modeling and intervention. F Thermal imaging photos of nude mice injected with different samples through the tail vein with the irradiation of 980 nm laser (n = 5 mice). G Temperature curves of the tumor of different groups (Data are presented as mean ± SD, n = 5 mice (for Laser group, n = 4 mice), one-way ANOVA multiple comparison test, the absence of a P-value signifies no statistical significant difference between the groups (P > 0.05)). H The Y content in the tumors of different groups (Data are presented as mean ± SD, n = 3 mice, one-way ANOVA multiple comparison test, the absence of a P-value signifies no statistical significant difference between the groups (P > 0.05)). I H&E staining of the tumor tissues of MCNs/Ln/GD and MCNs/Ln/GD/FR groups (n = 3 mice). J Histogram of the temperature of different organ tissues obtained from different groups (Data are presented as mean ± SD, n = 3 mice, one-way ANOVA multiple comparison test, the absence of a P-value signifies no statistical significant difference between the groups (P > 0.05)). K The Y content in different organ tissues in MCNs/Ln/GD/FR groups (Data are presented as mean ± SD, n = 4 mice, one-way ANOVA multiple comparison test, the absence of a P-value signifies no statistical significant difference between the groups (P > 0.05)). L Photos of nude mice in different time intervals after treatment. M Tumor volume change curves of the different groups (Data are presented as mean ± SD, n = 3 mice, one-way ANOVA multiple comparison test, the absence of a P-value signifies no statistical significant difference between the groups (P > 0.05)). N Photograph and qualities of the resected tumor tissues in different groups (Data are presented as mean ± SD, n = 3 mice, one-way ANOVA multiple comparison test, the absence of a P-value signifies no statistical significant difference between the groups (P > 0.05)).
