Fig. 6: flr-4(n2259) worms induce SARM1/TIR-1 phase transition. | Nature Communications

Fig. 6: flr-4(n2259) worms induce SARM1/TIR-1 phase transition.

From: Methionine cycle in C. elegans serotonergic neurons regulates diet-dependent behaviour and longevity through neuron-gut signaling

Fig. 6

A The flr-4(n2259) worms displayed increased TIR-1 puncta formation in comparison to wild-type worms. The red fluorescence channel images captured both TIR-1::wrmScarlet fluorescence and autofluorescence signals, while the green channel images exclusively revealed signals originating from the autofluorescent gut granules (Scale bar 10 μm). TIR-1::wrmScarlet puncta are denoted by arrowheads in the magnified images (Scale bar 2 μm). B Quantification of (A) and (Supplementary Fig. S8A). Each data point is the number of TIR-1::wrmScarlet puncta per worm. P-value determined using One-way ANOVA with Tukey’s multiple comparisons test. C Model showing TIR-1 oligomerization leading to activation of the p38-MAPK pathway mediated by B12-rich diet in the flr-4(n2259) worms. Created in BioRender. Mukhopadhyay, A. (2025) https://BioRender.com/83r2ngb. All experiments were performed at 20 °C. All data and analysis are provided in the Source Data file. Created with BioRender.com.

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