Fig. 3: Effects of the α-hydroxylated acid derivatives on Arabidopsis thaliana growth and validation of targeting AtDHAD.
a Col-0 seedlings were grown in soil in pots and treated with AA, I-6c, I-6e, or I-6g at a dose of 200 μg/mL by foliar spray. b Fresh weight inhibition rates of A. thaliana plants 20 days after treatment with AA, I-6c, I-6e, or I-6g. The data are presented as the mean ± SD. One-way analysis of variance was used, and the data for the test compounds were compared with those of AA. Three independent replicates were performed. c Wild-type A. thaliana (Col-0) seedlings were grown on vertically oriented 1/2 MS media alone or media supplemented with 25 μg/mL BCAAs and treated with either 50 μg/mL AA or I-6g. Control seedlings were treated with 50 μg/mL DMSO. All the seedlings were grown under etiolation conditions for 7 days. d KEGG enrichment analysis of differentially expressed genes in the leaves of A. thaliana after treatment with I-6g. The vertical axis displays the pathway name, and the horizontal axis presents the Rich factor (sample number/background number). The greater the Rich factor is, the greater the degree of enrichment. Generally, the FDR ranges from 0–1, and the closer it is to zero, the more significant the enrichment. Kd values of I-6e (e), AA (f) and TA (g) binding to AtDHAD. Each set of data comprises three replicates. R2 is the correlation coefficient, and R2 values closer to 1 represent a better fit. The data are presented as the mean ± SD. Source data are provided as a Source Data file.
