Fig. 2: Stress does not alter vCA1-vSUB basal excitatory synaptic strength in males.

a Electrically evoked EPSCs from vCA1 were recorded in vSUB neurons 24 h after stress in male mice (PND42-60). b Representative traces of firing patterns in burst-spiking (blue) or regular-spiking (red) cells. c, g Experimental schema to record EPSCs from burst-spiking or regular-spiking cells. d Input-output representative traces (left), summary graph (middle), and slope (right) for EPSCs recorded in burst-spiking (Stim Intensity x Stress Group F(4, 52) = 0.4200, p = 0.7934; slope, p = 0.6195 (t = 0.5234, df = 6); control n = 6/3, stress n = 8/3) or (h) regular-spiking cells (Stim Intensity x Stress Group F(4, 88) = 0.3494, p = 0.8438; slope, p = 0.8081 (t = 0.2458, df = 22); control n = 13/3, stress n = 11/4). e, i Representative traces of strontium-evoked asynchronous EPSCs (aEPSCs) and aEPSC amplitude summary graph (right) for burst-spiking ((e) p = 0.7469 (t = 0.3458, df = 4); control n = 17/3, stress n = 14/3) or regular-spiking cells (i; p = 0.9882 (t = 0.01494, df = 26); control n = 14/3, stress n = 14/3). f, j Representative PPR traces (50 ms) (left) and PPR measurements (right) from burst-spiking ((f) ISI x Stress Group F(4, 132) = 1.257, p = 0.2901; control n = 17/5, stress n = 18/4) or regular-spiking cells ((j) ISI x Stress Group F(4, 140) = 0.9487, p = 0.4378; control n = 19/6, stress n = 18/4). Data are mean ± SEM calculated from the total number of cells indicated in the graphs. Numbers in the legend represent the numbers of cells/animals. Statistical significance was determined by 2-way repeated measures ANOVA followed by Šidák’s multiple comparisons test ((d) (middle), (f, h) (middle), (j)) or a nested unpaired Student’s t-test (two-tailed) (d) (right), (e, h) (right), (i)). Source data are provided as a Source Data file.