Fig. 4: Stress weakens parvalbumin-positive interneuron (PV)-burst-spiking (BS) inhibition via decreased postsynaptic strength in males. | Nature Communications

Fig. 4: Stress weakens parvalbumin-positive interneuron (PV)-burst-spiking (BS) inhibition via decreased postsynaptic strength in males.

From: Acute stress causes sex-specific changes to ventral subiculum synapses, circuitry, and anxiety-like behavior

Fig. 4: Stress weakens parvalbumin-positive interneuron (PV)-burst-spiking (BS) inhibition via decreased postsynaptic strength in males.The alt text for this image may have been generated using AI.

a A Cre-dependent ChIEF adeno associated virus (AAV) was injected into vSUB of PV-Cre male mice (PND42-60) and optogenetically evoked inhibitory postsynaptic currents (IPSCs) from PV interneurons were recorded from burst-spiking (blue) or regular-spiking (red) cells 24 h after stress. b Example image of ChIEF-mRuby expression in the vSUB. c, g Optogenetically stimulated IPSCs were recorded in burst-spiking or regular-spiking cells after stress. d, h Input-output representative traces (left), summary graph (middle), and slope (right) for IPSCs recorded in burst-spiking ((d) LED Intensity x Stress Group F(3, 84) = 9.405, ****p < 0.0001, 0.213 mW, p = 0.8332; 0.518 mW, p = 1994; 1.050 mW, ***p = 0.0005; 1.640 mW, ****p < 0.0001; slope, *p = 0.0369 (t = 3.080, df = 4); control n = 15/3, stress n = 15/3) or regular-spiking cells (h; LED Intensity x Stress group F(3, 69) = 0.2209, p = 0.8815; slope, p = 0.6163 (t = 0.5424, df = 4); control n = 10/3, stress n = 15/3). e, i Representative traces of strontium-mediated asynchronous IPSCs (aIPSCs) after optogenetic stimulation (left) and aIPSC amplitude (right) for burst-spiking ((e) *p = 0.0109 (t = 2.690, df = 35); control n = 23/3, stress n = 14/3) or regular-spiking cells (i; p = 0.7594 (t = 0.3085, df = 38); control n = 19/3, stress n = 21/3). f, j Representative PPR traces (50 ms) (left) and PPR measurements (right) from burst-spiking ((f) p = 0.4417 (t = 0.8531, df = 4); control n = 21/3, stress n = 20/3) or regular-spiking cells ((j) p = 0.2826 (t = 1.093, df = 31); control 13/3, stress n = 20/3). Data are mean ± SEM calculated from the total number of cells indicated in the graphs. Numbers in the legend represent the numbers of cells/animals. Statistical significance was determined by a 2-way repeated measures ANOVA followed by Šidák’s multiple comparisons test ((d) (middle), (h) (middle)) or nested unpaired Student’s t-test (two-tailed) ((d) (left)-(f, h) (left)-(j)). Source data are provided as a Source Data file.

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