Fig. 4: The N-IDR ensures an adequate degree of disassembly for FtsZ protofilaments.

a Representative light scattering curves show time-dependent polymerization processes for indicated FtsZ forms, each with a starting monomeric concentration of 15 μM, initiated by 1 mM GTP, and monitored by 90° angle light scattering. b Coomassie brilliant blue staining shows the sedimentation analysis of polymerized FtsZ forms, with a starting monomeric concentration of 1 μM and 2 mM of GTP; T total, S supernatant, P pellet. c Statistically relative FtsZ proportions in pellets analyzed in (b); n = 3; mean ± SEM; parametric unpaired two-tailed Student’s t-test, ****P < 0.0001. d Negatively-stained electron micrographs and hand-drawn diagrams (beneath) of indicated FtsZ forms with a starting monomeric concentration of 1 μM; scale bar = 100 nm. e These curves reflect time-dependent relative Pi concentrations released from polymerization samples in (a). f Relative GTPase activity for indicated FtsZ forms with a starting monomeric concentration of 15 μM and addition of 1 mM GTP; n = 3, mean ± SEM; parametric unpaired two-tailed Student’s t-test, ***P < 0.001; P values for the different parameters are: FtsZ^WT-FtsZ^D10F = 0.0003; FtsZ^WT-FtsZ^∆NIDR = 0.0001. g Sedimentation analysis of indicated FtsZ forms with a starting monomeric concentration of 5 μM in the presence of 5 μM MinC. h Statistically relative FtsZ proportions in pellets in the presence of MinC, as analyzed in (g); n = 3, mean ± SEM; parametric unpaired two-tailed Student’s t-test, **P < 0.01, ****P < 0.0001; P value for FtsZ^WT-FtsZ^D10F = 0.0027. Source data of a–c, e–h are provided in the Source Data file.