Fig. 3: Computationally guided screen of lasso peptide RREs. | Nature Communications

Fig. 3: Computationally guided screen of lasso peptide RREs.

From: Characterizing and engineering post-translational modifications with high-throughput cell-free expression

Fig. 3

a Overall prediction and screening workflow for lasso peptide BGCs. b For predicted BGCs with a single RRE and precursor peptide, individual PUREfrex reactions expressing RREs and the respective peptide were cross-titrated and assessed for binding activity via AlphaLISA. c For predicted biosynthetic gene clusters with multiple RREs and precursor peptides, all possible combinations of RRE and precursor peptide were assessed for binding activity via AlphaLISA (select combinations shown, see Supplementary Fig. 6 for additional pairwise combinations). All data shown are single replicate, with confirmation reactions performed in biological triplicate provided in Supplementary Fig. 7 to show reproducibility. RLU relative luminescence units. Source data are provided in the Source Data 1 file.

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