Fig. 5: Cell-free workflow identifies high-efficiency CjPglB mutants. | Nature Communications

Fig. 5: Cell-free workflow identifies high-efficiency CjPglB mutants.

From: Characterizing and engineering post-translational modifications with high-throughput cell-free expression

Fig. 5

a Homology model of CjPglB94. Sites chosen for site saturation mutagenesis are highlighted in colors denoted in (b). b Rationale of sites chosen for mutagenesis. c AlphaLISA results for IVG reactions containing crude membrane fraction enriched with CPS from S. pneumoniae serotype 4, sfGFP with a 6xHis tag and DQNAT sequon, and a unique CjPglB mutant. Data are a mean of AlphaLISA signals produced by duplicate IVG reactions (n = 2). RLU relative luminescence units. Source data are provided in the Source Data 1 file.

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