Fig. 2: Structure of Pol I paused by an Ap site at i + 1. | Nature Communications

Fig. 2: Structure of Pol I paused by an Ap site at i + 1.

From: Cryo-EM uncovers a sequential mechanism for RNA polymerase I pausing and stalling at abasic DNA lesions

Fig. 2

a Diagram of the nucleic acid scaffold with an Ap site at i + 1. Filled squares denote nucleotides visible in the cryo-EM map that were modelled. b Consensus Ap-PEC structure with subunits colored as in (c) and close-up view of the active center. c Two views of the Closed Ap-PEC structure indicating the different subunits and structural elements in the enzyme. d Superposition between Closed Ap-PEC (colors) and Pol I with undamaged DNA in a post-translocated state (PDB code 5M3F, grey). e Superposition of Closed Ap-PEC (colors) with Pol I stalled at a CPD lesion (PDB code 6H67, grey and CPD lesion in yellow). f Comparison between Closed (grey) and Open (blue) Ap-PEC structures in two different views.

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