Fig. 2: Conservation of age-related ubiquitylation changes in killifish and mouse.
From: Aging and diet alter the protein ubiquitylation landscape in the mouse brain
A Mapping of ubiquitylation sites between M. musculus and N. furzeri. The percentage of consistently up (red and light red for P < 0.05 or P < 0.25, respectively, in both datasets), down (blue and light blue for P < 0.05 or P < 0.25, respectively, on both datasets), and not consistently (gray; P < 0.25) regulated sites are shown in the pie plot (N = 5 for mouse; N = 4 for young, and N = 3 for old killifish; biological replicates). B Barplot showing correlation between age-related changes in ubiquitylated peptides (purple bars) and protein abundance (gray bars) in mouse and killifish. Two-sided Pearson’s correlation test. On the y-axis, different P thresholds are applied. C Heatmap of age-affected ubiquitylated sites conserved in mouse and killifish. Lysine position refers to the mouse protein sequence. Fold changes have been corrected for protein abundance (P < 0.05 in at least one species). D–G Examples of peptides that show age-related changes of ubiquitylation independently of protein abundance, both in mouse and killifish. The flanking sequence (FS) of the modified peptide residues is indicated under the protein name (N = 5 for mouse, N = 4 for young, and N = 3 for old killifish, biological replicates. Q values from Spectronaut differential abundance analysis, data shown as averages ± SD). *Q/Adj.P ≤ 0.05; **Q/Adj.P ≤ 0.01, ***Q/Adj.P ≤ 0.001, ****Q/Adj.P ≤ 0.0001. Source data are provided as a Source Data file. Related to Fig. S5 and Supplementary Data 3.
