Fig. 4: Quantification of ubiquitin-chain linkages in mouse brain and iNeurons.
From: Aging and diet alter the protein ubiquitylation landscape in the mouse brain
A Experimental scheme for absolute quantification of ubiquitin chains and the total ubiquitin pool (N = 5 for mouse, N = 3 for iNeurons, biological replicates). Created in BioRender. Ori, A. (2025) https://BioRender.com/2947h3v. B Percentage of total and polyubiquitin in mouse brain aging and in iNeurons upon drug treatments measured via AQUA-PRM, * refers to unpaired t-test, data shown as averages ± SD. C Barplot of total ubiquitin, linear (M1), and branched lysine chains absolute quantification in young and old mice brain and in iNeurons (N = 5 for mouse, N = 3 for iNeurons, biological replicates, * refers to unpaired t-test, One-way ANOVA results are indicated above the barplots, data shown as averages ± SD. *P ≤ 0.05; **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001. Source data are provided as a Source Data file. Specific P values are listed in Supplementary Data 8. Related to Supplementary Data 6.
