Fig. 2: Establishing a Crabtree-negative strain based on the sucrose phosphorolysis-capable strain. | Nature Communications

Fig. 2: Establishing a Crabtree-negative strain based on the sucrose phosphorolysis-capable strain.

From: Sucrose-driven carbon redox rebalancing eliminates the Crabtree effect and boosts energy metabolism in yeast

Fig. 2: Establishing a Crabtree-negative strain based on the sucrose phosphorolysis-capable strain.The alternative text for this image may have been generated using AI.

a Comparison of ethanol production capacity and NADH/NAD+ ratio between the sucrose phosphorylase and glucose metabolism models. b Comparison of growth between the sucrose phosphorolysis and glucose metabolism models. c Functional schematic diagram of inositol pyrophosphatase OCA5 regulates glycolysis and respiration by adjusting levels of 5-diphosphoinositol 1,2,3,4,6-pentakisphosphate (5-InsP7). d Growth of the OCA5Δ strain under different metabolic conditions, with ZQS15 as the control. e Accumulation of ethanol and glycerol in the OCA5Δ strain under sucrose phosphorolysis metabolism mode. f Comparison of the NADH/NAD+ ratio between the OCA5Δ strain and the wild-type strain under sucrose phosphorolysis metabolism mode. g Schematic diagram of metabolic changes in the PGI1Δ strain under sucrose phosphorolysis mode. h Ethanol accumulation in PGI1Δ strains compared to PGI1 wild-type strains. ZQS17 and ZQS18 are PGI1Δ strains, while ZQS15 and ZQS16 are control strains. i Glycerol production in PGI1Δ strains compared to PGI1 wild-type strains. j Changes in the PGI1Δ strain’s NADH/NAD+ ratio. k Impact of PGI1 knockout on the growth cycle of different strains. All data are expressed as mean ± SD of biological triplicates. Statistical analysis was conducted using the Student’s t-test (two-tailed; sample size, n = 3). Source data are provided as a Source Data file.

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