Fig. 4: Excavation and identification of growth-regulated genes.
a Grouping of different comparative strains. The genotypes of strains: ZQS16 (OCA5Δ), ZQS17 (PGI1Δ), ZQS18 (OCA5Δ; PGI1Δ). Comparison I and Comparison IV for sucrose phosphorolysis metabolism mode versus glucose metabolism mode. Comparison II and Comparison V were Crabtree-positive strains in the glucose metabolism pattern versus Crabtree-negative strains in the sucrose metabolism pattern. Comparison groups III and VI were Crabtree-positive versus Crabtree-negative strains in sucrose metabolism patterns. b Venn plots showing genes in the three comparison groups of the OCA5 knocked-out strains (Comparisons I, II, and III). c Venn plots showing genes in three comparison groups of OCA5 wild-type strains (Comparisons IV, V, and VI). d Heatmap illustrating the upregulation and downregulation of transcription levels for the selected 26 genes across different comparison groups. The log2 fold change of genes (down/up) is represented by blue (down) and red (up). The intensity of the red colour indicates the degree of upregulation, with darker shades indicating more pronounced upregulation. Conversely, darker shades of blue indicate a more pronounced downregulation of genes. e Impact of overexpression or knockout of 26 potential growth-regulating genes on the lag phase of the strains. The untreated strain ZQS18 was the control. f Maximum OD600 for strains with effectively shortened lag phase time. ZQS18 was the control strain. g Comparison of lag phase duration across strains with different gene knockout or overexpression combinations. The strains with single-factor treatment were controls. All data are expressed as mean ± SD of biological triplicates. Statistical analysis was conducted using the Student’s t-test (two-tailed; sample size, n = 3). Source data are provided as a Source Data file.
