Fig. 1: Mag-Net affinity capture workflow.
From: Enrichment of extracellular vesicles using Mag-Net for the analysis of the plasma proteome
a SAX magnetic beads are incubated with plasma to sieve and bind negatively charged membrane-bound particles. The beads are immobilized with a magnet, and abundant plasma proteins are gently washed away. The membrane particles are lysed on the beads, and proteins are reduced and alkylated, precipitated back onto the beads, and then digested with trypsin. Peptides are collected and analyzed using nLC-MS/MS. b The Kingfisher robot method to automate the Mag-Net workflow.
