Fig. 2: cTAGE5 ablation in APCs reduces the size of adipocytes in WAT and decreases lipid accumulation in BAT.

a H&E staining of P8 iWAT sections (Ctrl and AP-cKO, n = 3 biologically independent samples). Scale bar, 5 µm. b, c Adipocyte area distribution (b) and average adipocyte area (c) from P8 iWAT sections were calculated using Adiposoft software (Ctrl and AP-cKO, n = 3 biologically independent samples). d H&E staining of P8 BAT sections (Ctrl and AP-cKO, n = 3 biologically independent samples). Scale bar, 50 µm. e–g Levels of TG, TC, and glycerol in serum from P8 control and AP-cKO mice (Ctrl, n = 6, 6, 8 biologically independent mice in (e–g), respectively; AP-cKO, n = 4, 5, 6 biologically independent mice in (e–g), respectively). h H&E staining of P16 iWAT sections (Ctrl and AP-cKO, n = 3 biologically independent samples). Scale bar, 5 µm. i, j Adipocyte area distribution (i) and average adipocyte area (j) from P16 iWAT sections were calculated as in (b, c) (Ctrl and AP-cKO, n = 3 biologically independent samples). k H&E staining of P16 BAT sections (Ctrl and AP-cKO, n = 3 biologically independent samples). Scale bar, 50 µm. l–n Levels of TG, TC, and glycerol in serum from P16 control and AP-cKO mice (Ctrl, n = 4, 4, 8 biologically independent mice in (l–n) respectively; AP-cKO, n = 4, 4, 7 biologically independent mice in (l–n), respectively). a–n Representative of three independent experiments. All data are mean ± s.e.m. *P < 0.05, **P < 0.01, and ***P < 0.001. Unpaired two-tailed Student’s t-test (g, j) or two-way ANOVA followed by Bonferroni post hoc test (i). Source data with exact P-value are provided as a Source Data file.