Fig. 5: cTAGE5 deletion in APCs enhances lipolysis in WAT adipocytes.

a GO analysis of significantly differentially expressed proteins in iWAT from P8 control and AP-cKO mice. Proteins related to lipid catabolic processes were upregulated in cTAGE5 AP-cKO iWAT (Ctrl and AP-cKO, n = 3 biologically independent mice). Functional enrichment analysis for GO terms was performed using the hypergeometric test, followed by Benjamini–Hochberg correction to control the false discovery rate (FDR). b mRNA levels of Atgl, Hsl, and Mgl in P8 iWAT (Ctrl and AP-cKO, n = 6 biologically independent samples). c, d mRNA levels of Atgl, Hsl, and Mgl in SVFs isolated from P8 iWAT after inducing adipocyte differentiation for 6 days (c) and 12 days (d) (Ctrl and AP-cKO, n = 3 biologically independent samples). e 10–15 mg iWAT fat pads isolated from P8 mice were stimulated with 250 µl of 2% free fatty acid BSA in the presence or absence of 10 µM CL 316,243 (CL) for 2 hours at 37 °C. Levels of released glycerol in medium were analyzed (Ctrl and AP-cKO, n = 3 biologically independent mice). f TEM of iWAT from P16 mice (Ctrl and AP-cKO, n = 3 biologically independent mice). LD, lipid droplet; N, nucleus. Scale bar, 1 µm. g Western blotting and quantitation analysis of cTAGE5 and ATGL in P8 iWAT (Ctrl and AP-cKO, n = 6 biologically independent samples). h Primary P8 SVFs were treated with Vehicle (Veh, DMSO), 5 μM atglistatin (Atgli) on day 3 after adipogenic cocktail treatment, 1 μM LatB for 2 hours on day 0 and 3 after adipogenic cocktail treatments, or 1 μM LatB concurrently with 5 μM Atgli. Oil Red O staining and quantification of SVFs after inducing adipocyte differentiation for 10 days (Ctrl and AP-cKO, n = 4 biologically independent samples). Scale bars, 50 μm. i Mice were intraperitoneally injected with Veh (DMSO) or 200 μM/kg Atgli at P8, P11, and P14. Survival of different groups was monitored (Ctrl+Veh, AP-cKO+Veh, and AP-cKO+Atgli, n = 20). j, k Body weight (j) and iWAT, eWAT, and BAT (k) of mice treated with Veh (DMSO) or Atgli at P16. The ratio to body weight was measured (k) (Ctrl and AP-cKO, n = 4 biologically independent mice). b–e, g, j–k Representative of three independent experiments. h Representative of four independent experiments. All data are mean ± s.e.m. *P < 0.05, **P < 0.01, and ***P < 0.001. Unpaired two-tailed Student’s t-test (b–e, g) or one-way ANOVA followed by Bonferroni post hoc test (h, j, k) or log-rank test (i). Source data with exact P-value are provided as a Source Data file.