Table 1 Products of in vitro activity assays on peplusol and squalene synthases and heptaswap mutants

From: Evolution of linear triterpenoid biosynthesis within the Euphorbia genus

 

AtSS

EpSS-L2

EpSS-L1

EpSS-L1toL2

EpSS-L2to-L1

Empty vector

1

2

7

1

2

7

1

2

7

1

2

7

1

2

7

1

2

7

pH 6.5

NADPH, Mg2+, FPP

-

++++

-

-

++++

-

++

-

++

++

++

-

-

+

-

-

-

-

NADP + , Mg2+, FPP

-

++++

-

-

+++

-

+

++

+

+

-

-

-

-

-

-

-

-

Mg2+, FPP

-

+++

-

-

+++

-

+

++

+

+

-

-

-

-

-

-

-

-

FPP

-

-

-

-

-

-

++

-

-

-

-

-

-

+

-

-

-

-

NADPH, Mg2+

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

pH 7.5

NADPH, Mg2+, FPP

-

++++

-

-

++++

-

++

+

++

+

++

-

-

-

-

-

-

-

NADP + , Mg2+, FPP

-

++++

-

-

++++

-

++

+

+

++

++

-

-

+

-

-

-

-

Mg2+, FPP

-

+++

-

-

++

-

+

-

-

+

-

-

-

-

-

-

-

-

FPP

-

+++

-

-

-

-

++

-

-

-

-

-

-

-

-

-

-

-

NADPH, Mg2+

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

  1. 1: peplusol (retention time − 22.86 min); 2: squalene (retention time − 22.04 min); 7: unknown (retention time − 21.92 min). Signal intensity for m/z 69 ions: “++++”-very high (1.1E06-4E06); “+++”-high (9E04-7.7E05); “++”-low (1E04-7.8E04); “+”-very low (3.2E03-9.8E03); “-”-not detectable (0) as detailed in Supplementary Fig. 8.
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