Table 3 Estimated binding affinities of the natural variants of FMN aptamers with FMN and synthetic ligand (DHEF) by fluorescence-titration binding assay

From: Rational development of FMN-based orthogonal riboswitch that functions in response to specific non-cognate ligand

 

Nucleotide positionsa

KD

  

FMN

DHEF

Aptamers

G10

G11

G12

G47

G62

G84

[×10−8 L mol−1]

[×10−8 L mol−1]

HO

G7

G8

A9

A45

G65

G87

NB

NB

AS

G7

G8

G9

G44

G72

A94

6.72 ± 0.27

NB

CB

G7

G8

G9

G44

G58

A80

10.6 ± 0.65

NB

MS

G7

G8

G9

G55

G94

A116

3.66 ± 0.10

NB

Csp

A10

A11

A12

A45

A60

G82

NB

NB

M11b

G7

A8

A9

G57

A74

G96

NB

2.72 ± 0.15

M15b

G7

A8

A9

G57

A74

A96

NB

1.58 ± 0.27

  1. NB no binding.
  2. aNucleotides are numbered in accordance with the consensus FMN aptamer from Fusobacterium nucleatum. The analogous nucleotides are mentioned for each aptamer individually in the table.
  3. bKD values are the same as mentioned in Table 1.
Back to article page