Fig. 3: Early SN-ROP-defined redox patterns distinguish CAR-T cell states across time. | Nature Communications

Fig. 3: Early SN-ROP-defined redox patterns distinguish CAR-T cell states across time.

From: Single-cell signaling network profiling during redox stress reveals dynamic redox regulation in immune cells

Fig. 3

a Diagram of the experimental protocol. Blood was sampled from leukemia patients (n = 7) undergoing CAR-T therapy on days 0, 7, 14, 21, 28, and 90 after CAR-T infusion. CD8+ T cells were subsequently isolated and analyzed using SN-ROP. Created with BioRender.com and used with permission under an Academia Sinica institutional publication license. b tSNE plot of CAR-positive T cells, clustered based on their redox profiles using FlowSOM. c ASINH-transformed expression levels of SN-ROP markers for active (yellow) and basal (red) clusters. Each box represents the distribution of single-cell expression values derived from 7 biologically independent CAR-T cell patient samples. Box plots display the median (center line), interquartile range (IQR; box limits), and whiskers extending to 1.5×IQR. Minima and maxima beyond the whiskers are shown as individual points (outliers). No statistical comparisons were performed. d Correlation heatmap of all clusters across all sample collection time points. Upper square marks the correlated basal type and the lower right square denotes the active type detected at the 90-day time point. Blue squares highlight biotin+ (i.e., CAR-T-positive) cells at 90 days post-infusion.

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