Fig. 2: Single cell transcriptomics identifies melanocyte/neural crest-derived, macrophage, and fibroblast populations in normal skin and Braf CA/+ and Braf CA/+; PtenΔ/+ tumors.
a Coat colors, genotypes and numbers of mice subjected to single-cell RNA sequencing. Created in BioRender. Shiu, J. (2025) https://BioRender.com/2832vxc. b 345,427 cells from 36 mice (47 normal, nevus-containing skin, and melanoma samples) from the genotypes in panel A were subjected to scRNAseq, jointly clustered, and projected onto a common UMAP. ScRNA-seq identified populations enriched in tumors, highlighted in the boxed areas. IRS: Inner root sheath. HF: Hair follicle. EC: Endothelial cell. c NC-derived clusters (identified by the expression of S100b, Dct, Mpz [as well as a Cre-reporter gene in selected cases]) were further subsetted (35,527 cells in total) and populations specific to tissue and coat color were characterized. d Differentially expressed genes associated with NC-derived clusters defined in (c). Known melanoma markers are labeled in black; colored bars and other text colors correspond to labeling in (c). e Gene expression profiles of tumor-enriched fibroblasts (purple), macrophages (brown) and tumor cells (pink) compared among the tumor genotypes. Each column in the heatmap represents a cell from the corresponding group.
