Fig. 4: Signatures of NC-derived populations conserved in mouse and human melanoma.
a The tissue source distribution of NC-derived clusters from three genotypes: Albino Braf CA/+ (21,600 cells), Albino Braf CA/+; PtenΔ/+ (5973 cells), and Black Braf CA/+; PtenΔ/+ (3926 cells), is shown. Prolif: proliferating. b−d Every cell was assigned a score for how well its gene expression fits a published gene expression signature18,22,55, relative to the other NC-derived cell types (see Methods and Supplementary Data 24) and these were overlayed on the UMAPs for each of the tumor genotypes (color bar denote membership agreement level; red=strong agreement; blue = poor agreement). Gene signatures that aligned primarily with principal tumor cells are shown in (b) those that aligned strongly with LNM cells are shown in (c) and those that aligned with melanocytes are shown in (d). Black dashes outline cell-types in the individual datasets. e Feature plots of expression of selected pigmentation genes in the three tumor datasets. EMT: Epithelial-mesenchymal transitions. NCSC Neural crest stem-cell, Sig signature. Color bar denotes expression level.
