Fig. 6: Local immunity activation by radiotherapy-activated O-IMDQ decreases cold tumor metastasis.
a Six to 8 weeks old female C57BL/6J mice (n = 6 mice) were inoculated with 2.5 × 105 B16F10 cells. After 1 week, 2.5 × 106 B16/F10 cells were intravenously injected. Tumor-bearing mice were i.v. treated with O-IMDQ and/or 6 Gy radiation on days 8, 11, and 14. The local tumor volume was measured. b The photos of lung metastasis. c The colony number on the lung was counted. d Six to 8 weeks old Balb/c mice (n = 8 mice) were inoculated with 2 × 105 4T1-luc2 cells. Tumor-bearing mice were i.v. treated with O-IMDQ and/or 6 Gy radiation on days 10, 13, and 17. The metastasis of the lung was measured by the IVIS Spectrum. Scale bar = 50 um. e The counts on the lung was counted (n = 8 mice). f Survival curve was shown. Data were represented as means ± SEM and are representative of at least two independent experiments. Statistical analysis was performed using Student’s two-tailed unpaired t-test (c) or two-way ANOVA (a, e). Exact P value has been provided in figures. N numbers define the biological replicates. Source data are provided as a Source Data file.
