Fig. 2: Impaired condensate growth rates and sizes resulting from homogenized chromatin network.

a Schematics of Corelet system adapted from Bracha et al.⁵². b Representative fluorescence images of Corelet expressed in U2OS cells. Representative images of FUS_N Corelets in U2OS cells before and after TSA treatment, with 15 s (c) or 180 s (d) of light activation. Averaged condensate diameter per U2OS cell before (n = 42) and after TSA treatment (n = 20), with 15 s (e) or 180 s (f) of light activation. Distribution of condensate diameter in U2OS cells before (n = 42) and after TSA treatment (n = 20), with 15 s (g) or 180 s (h) of light activation. i Number of condensates in U2OS cells under TSA-treated and untreated conditions, with 180 s of light activation. j Mean diameter of condensates in U2OS cells before and after TSA treatment, with 180 s of light activation. k Mean diameter of condensates during coarsening in U2OS cells before and after TSA treatment. The data is well-fit by a power law (dashed line). Black solid lines are visual guides. In (i, j, k), s.e.m. is represented by the shaded area. n = 19 cells. Representative images (l) and mean nuclear speckle area per cell (m) of HEK cells with tagging of eYFP-SRRM2 at the endogenous locus via CRISPR/Cas9. Cells are imaged 0 h and 28 h with and without TSA treatment. n = 268, 135, 257, 74 cells from 4 biological replicates for Ctrl (0 h), Ctrl (28 h), TSA (0 h) and TSA (28 h), respectively. In the box plots (d, g, m), 25%, 50%, and 75% of the data are indicated using hinges and center lines. The whiskers extend to the highest and lowest values. In (d, g, m), p > 0.05 is non-significant. * for p < 0.05, ** for p < 0.01, *** for p < 0.001, and **** for p < 0.0001 based on two-sided student’s t-test. For (b, f, l), the experiment was repeated three times independently with similar results. For (d, e, g, h, i, j, k), three biological replicates are used for statistical analysis.