Fig. 4: Structure and phenotypical analysis of PitA and TesB mutation.
From: In-host evolution of Yersinia enterocolitica during a chronic human infection
a, c Domain analysis of Y11 PitA and TesB proteins respectively, as found on InterPro on 2025.02.12. Mutation position is shown by a vertical magenta line and residues from the catalytic triad or dimer interface close to the TesB mutation are annotated. Domains are annotated with entry numbers for InterPro (IPR), NCBIfam (NF or TIGR), PANTHER (PTHR) Pfam (PF), CATH-Gene3D (G3DSA), SUPERFAMILY (SSF) or CDD (cd). Non cytoplasmic, cytoplasmic and transmembrane regions are annotated via Phobius and Transmembrane Helix are annotated via TMHMM. AlphaFold confidence (pLDDT) is colored from very low confidence (orange) to very high confidence (dark blue). b AlphaFold prediction structure for PitAYe.1 (left) and PitAYe.3 (right). Truncated residues are displayed with the same color code as the regions in panel (a). d Structure of the TesB active site of E. coli with a G280S mutation, annotated in magenta, showing an additional interfering hydrogen bond from the serine side chain. Carbon atoms are shown in green, oxygen atoms in red, nitrogen atoms in blue and hydrogen atoms in white. e Thioesterase II activity of IP29610 and Ye.1 mutant harboring different tesB alleles (IP29610 allele in orange and Ye.1 allele in blue), measured by the CoA release from decanoyl-CoA. Each point shows the mean and standard deviation of n = 3 replicated wells in one experiment. The green curve represents the negative control without substrate. Source data are provided as a Source Data file.
