Fig. 1: Growth of class IV neurons during development.
From: Neurons exploit stochastic growth to rapidly and economically build dense dendritic arbors
a Schematic of a Drosophila larva from the dorsal side attacked by a parasitoid wasp. Larval body plan axes are marked with anterior (A), posterior (P), dorsal (D), ventral (V), dorsal left (L), and dorsal right (R). The size of the larva is exaggerated in comparison to the wasp. b Class IV neurons (48 h after egg-lay, AEL) are marked with the transmembrane protein CD4 tagged with green fluorescent protein (GFP) (genotype is;;ppkCD4-tdGFP) and imaged using a 40x water immersion objective by spinning-disk confocal microscopy and displayed as the maximum projection of 10 sections (see Methods). The scale bar is 100 μm and also applies to (c–e). White arrowheads indicate tilings. c-e Coarse-grained dendrite length density of class IV neurons at 24 (13 cells from 10 animals), 48 (12 cells from 12 animals), and 96 (9 cells from 9 animals) hours AEL, respectively. The rectangles represent the corresponding average segment sizes at each developmental stage, with gray-shaded regions being the standard error of the mean (9 cells from 3 animals for each developmental stage). f, g Mean dendrite length density along the AP and LR axes at different developmental stages: 24 h in red, 48 h in purple, and 96 h in yellow. The scales in (f, g) are the same as for (b-e).
