Fig. 3: Substrate scope of photoenzyme catalysis.
From: Photoenzymatic enantioselective synthesis of fluorinated amides with remote stereocenter
Reaction conditions: fluorinated reagent: 10 mM, alkene: 5 mM, NADP+: 0.5 mM, glucose: 50 mM, GDH: 0.5 μg/μL, ene-reductase: 2 mol% based on fluorinated reagents, DMSO: 10% (v/v), glycerol: 10% (v/v), Tris-HCl (pH = 8.5): add to 800 μL. Reaction at room temperature for 16 h. †(Z)-But-2-en-2-ylbenzene was used as the alkene. *chiral carbon. The yields were determined by 1H NMR with 1,3,5-trimethoxybenzene as an internal standard. The ee values were calculated using the product peak area from chiral HPLC.
