Fig. 4: Investigation of the enzyme’s catalytic mechanism through mutagenesis and deuteration experiment.
From: Photoenzymatic enantioselective synthesis of fluorinated amides with remote stereocenter
a Investigation of the impact of three tyrosine residues (Y82/Y196/Y375) on the reaction process. b Deuterium labeling experiments. Track the source of chiral hydrogen atoms in the product and provide the evidence for the reaction mechanism (D-Tris: Tris+DCl+D2O, pH = 8.5).
