Fig. 1: ApoM therapy ameliorates a dry AMD-like phenotype.
A ApoM levels controlled to total cholesterol levels were measured in patients with (n = 53) or without AMD (n = 328) aged 65 and over. Patients with AMD had significantly lower ApoM levels compared to those without AMD (****p = 0.0000000006, two-tailed Welch’s t-test). B High fat diet fed Abca1/g1-rod/-rod mice were treated with either ApoMKO or ApoMTG plasma (created in BioRender. Lee, T. (2025) https://BioRender.com/li4w1zf). C RPE functional assessment using light bleach dark adaptation electroretinography (n = 6) revealed significantly improved functional recovery in ApoMTG plasma treated mice (*p = 0.0271, Two-way ANOVA). D, E Representative TEM imaging of RPE from either ApoMKO or ApoMTG plasma treated Abca1/g1-rod/-rod mice. Red arrows point to lipid droplets. F Quantitative assessment of lipid droplets per high-powered field within the RPE (n = 30) revealed significantly reduced lipid droplets in ApoMTG plasma treated mice (****p = 0.0000011, two-tailed Welch’s t-test). G Quantitative assessment of melanosomes per high-powered field within the RPE (n = 30) revealed significantly increased melanosome counts in ApoMTG plasma treated mice (****p = 0.0000068, two-tailed Welch’s t-test). Full-field scotopic electroretinography (n = 6) demonstrated improved H rod photoreceptor (*p = 0.0343, Two-way ANOVA) but not I inner retina (Two-way ANOVA) function in ApoMTG plasma-treated mice. J Representative traces of 0 dB intensity flashes show an improved waveform from ApoMTg+ injected mice. The scale bar represents 2 μm. Values are mean ± SE. Source data are provided as a Source Data file.
