Table 1 Thermodynamic parameters of ITC titrations

p53^{1-94 b}

FOXO4^FH

0.79 ± 0.17

2.5 ± 0.6

6.3 ± 0.3

−13.9 ± 0.5

p53^{1-94 I50A b}

FOXO4^FH

0.83 ± 0.15

6.3 ± 0.8

4.6 ± 0.7

−11.9 ± 0.5

p53^{1-94 W53A b}

FOXO4^FH

0.87 ± 0.10

8.2 ± 1.8

5.6 ± 0.4

−12.6 ± 0.2

p53^{1-94 F54A b}

FOXO4^FH

0.83 ± 0.05

5.1 ± 2.4

5.7 ± 0.5

−12.9 ± 1.0

p53^{1-94 IFWtoA b}

FOXO4^FH

0.95 ± 0.11

16.7 ± 9.2

6.4 ± 2.1

−13.0 ± 1.9

p53^{1-94 high salt}

FOXO4^FH

n.d.

n.d.

n.d.

n.d.

p53^{1-94 a}

FOXO4-DRI

0.90

0.4 ± 0.3

5.9 ± 0.1

−14.6

p53^{TAD2 a}

FOXO4^FH

n.d.

n.d.

n.d.

n.d.

p53^{TAD2pS46 a}

FOXO4^FH

1.48

30.6 ± 4.8

−1.6 ± 0.1

−5.0

p53^{TAD2pT55 a}

FOXO4^FH

0.95

4.6 ± 0.2

−3.1 ± 0.1

−4.2

p53^{TAD2pS46T55 a}

FOXO4^FH

1.09

5.7 ± 0.3

−4.4 ± 0.1

−2.7

p53^{TAD2 a}

FOXO4-DRI

0.90

18.5 ± 3.6

0.2 ± 0.1

−6.7

p53^{TAD2pS46 a}

FOXO4-DRI

0.71

10.5 ± 1.5

−1.8 ± 0.1

−5.0

p53^{TAD2pT55 a}

FOXO4-DRI

0.74

5.8 ± 1.2

−4.7 ± 0.1

−5.0

p53^{TAD2pS46T55 a}

FOXO4-DRI

1.11

4.9 ± 0.3

−4.5 ± 0.1

−2.6