Fig. 2: Single-cell level characterization of m6A.
From: Isoform characterization of m6A in single cells identifies its role in RNA surveillance
a Experimental workflow of m6A-isoSC-seq. b Scatter plot demonstrating the correlation of C-to-U mutation rate in bulk eDART-seq and pseudobulk of Illumina based single-cell data of HEK293T cells (n = 1584). Pearson correlation coefficient and P value based on a two-tailed t test were shown. c Volcano plot showing the correlation between m6A methylation level and gene expression at single-cell level in HeLa. Pearson correlation coefficient and P value based on a two-tailed t-test were shown. d Box plot demonstrating the correlation between normalized gene expression and the m6A-isoSC-seq determined m6A levels of FTH1 gene in single-cell level. P values of two-tailed Wilcoxon tests are indicated. e UMAP visualization of all cells clustered by MMRs in Illumina based single-cell data. f Heatmap of the MMRs showing top 50 differentially methylated marker peaks across different MMR classified cell clusters. g Stacked bar plot showing the percentages of cell types for each MMR classified cell cluster. The number of cells for the major cell type of each cluster is indicated in the corresponding area. h Distributions of the percentage of cells methylated in each mutation peak in HeLa, HepG2, and HEK293T, respectively. The methylated cell was defined as those cells with at least one C-to-U mutation in the peak. i Scatter plot demonstrating the correlation between the m6A methylation level of pseudobulk and the average m6A methylation levels of single cells for each mutation peak (n = 3161) in HEK293T. Pearson correlation coefficient and P value based on a two-tailed t-test were shown. j Scatter plot demonstrating the correlation between the m6A methylation level of pseudobulk and the percentage of methylated cells for each mutation peak (n = 2878) in HEK293T. Pearson correlation coefficient and P value based on a two-tailed t test were shown. For box plots, the horizontal line represents the median value, the lower and upper quartiles represent the 25th and 75th percentiles, and the whiskers are extended to 1.5 × IQR. Source data are provided as a Source Data file.
