Fig. 7: CMD contributes to m6A-mediated degradation of misprocessed isoforms.
From: Isoform characterization of m6A in single cells identifies its role in RNA surveillance
a CDS C-to-U mutation rates in IpA isoforms (exon number ≤ 4) and their corresponding protein-coding isoforms in the pseudobulk of ONT single-cell data. b Density plot showing the length of coding sequence in last exon for CDM targeted genes and CMD non-targeted genes, respectively. P value from two-tailed Wilcoxon test is indicated. c Comparing the percentages of isoforms with coding sequence in last exon longer than 200 bp of IpA isoforms (exon number ≤ 4) and their corresponding protein-coding isoforms in the same genes. d Comparing the percentages of CDS m6A sites in IpA isoforms with different lengths of coding sequence in last exon in HEK293T DRS data. Comparing the modification levels of CDS m6A sites in IpA isoforms (e) and canonical isoforms (f) with different lengths of coding sequence in last exon in the HEK293T DRS dataset. Comparing the expression changes of genes with different lengths of coding sequence in last exon in wild-type and YTHDF2 (g) or DCP2 (h) knockdown HeLa cells. i Comparing the percentages of DCP2-targeted genes across categories defined by the lengths of coding sequence in last exon. j Comparing the percentages of isoforms with different lengths of internal CDS exons between high methylated NMD isoforms and their corresponding protein-coding isoforms in the same genes. k Comparing the percentages of genes (coding regions in the last exons ≤ 200 nt) with different lengths of internal CDS exons between CMD target genes and CMD non-target genes. l Comparing the modification levels of CDS m6A sites in canonical protein-coding isoforms (coding regions in the last exons ≤ 200 nt) with different lengths of maximal internal CDS exons in the DRS dataset. For box plots, P values of two-tailed Wilcoxon test are indicated, the horizontal line represents the median value, the lower and upper quartiles represent the 25th and 75th percentiles, and the whiskers are extended to 1.5 × IQR. For stacked bar plot, P values of one-tailed Fisher’s exact test are indicated. Source data are provided as a Source Data file.
