Fig. 2: Surfactin is the major anti-microbial compound produced by TG1-2.
a The flow chart of TG1-2 culture filtrate inhibition assay (left panel) and the inhibitory effect on the growth of V. dahliae-V592 (right panel). The culture filtrate of DH5α and LB liquid medium serve as parallel controls. Figure created in BioRender. Duan, C. (2025) https://biorender.com/qnninz4. b Inhibitory activity of TG1-2 culture filtrate on the growth of V. dahliae-V592 mycelium in a co-culture assay. Untreated pathogens serve as negative controls. c Column diagram showing the diameter of the inhibition zone in (b). Data are the means ± SD (n = 3 biological independent repeats). Asterisk above the bars indicate significant differences among different treatments determined by unpaired one-sided t-test at ***P < 0.001, all ***P < 0.0001. d The proportion of strain TG1-2 metabolites in various chemical classifications. e Surfactin is highly expressed in TG1-2. The highly expressed compound was obtained through multiple analysis of significant differences in TG1-2 and DH5α metabolite expression. f Secondary spectra of surfactin b and surfactin c in mass spectrometry analysis. The signal with blue color in the upper is the spectra of surfactin detected in TG1-2, and the signal with red color below is the standard. g The anti-microbial activity of surfactin on V. dahliae-V592. The dissolution buffer for surfactin is water. The phenotype was recorded 5 days after culturing on a plate with a diameter of 6 cm. h Diameter of the inhibition zone of V. dahliae-V592 cultured on a PDA plate containing buffer (H2O), TG1-2cf, and surfactin. Data are the means ± SD (n = 3 biological independent repeats). Different letters above the bars indicate significant difference among different treatments determined by multiple comparisons based on one-way ANOVA (one-sided) followed by Scheffe correction at P < 0.05, P = 2.51 × 10−5, =1.08 × 10−4 in sequence. Source data are provided as a Source Data file.
