Fig. 3: NatA acetyltransferase complex functions in V. dahliae tolerance to TG1-2. | Nature Communications

Fig. 3: NatA acetyltransferase complex functions in V. dahliae tolerance to TG1-2.

From: Reduced fungal protein acetylation mediates the antimicrobial activity of a rhizosphere bacterium against a phytopathogenic fungus

Fig. 3

a Flow chart showing the T-DNA mutant screen strategy for sensitivity to TG1-2. T-DNA mutants were generated through G418 resistance and then screened on a plate containing TG1-2 culture filtrate. Figure created in BioRender. Duan, C. (2025) https://biorender.com/hiyv1b3. b A T-DNA mutant, Vdnat1-T, was hypersensitive to TG1-2cf. Photographs were taken 5 days post incubation. c The sensitivity of VdNat1 knockout mutant and overexpression strains to TG1-2cf. Photographs were taken 5 days post incubation. d The growth inhibition rate of VdΔnat1 and VdNat1OE was calculated after 5 days of incubation. Data are the means ± SD (n = 3 biological independent repeats). Different letters above the bars indicate significant difference as determined by multiple comparisons based on one-way ANOVA (one-sided) followed by Scheffe correction at P < 0.05, P = 6.42 × 10−4, =0.0046, =3.28 × 10−5 in sequence. e VdNat1 interacts with VdArd1 in the Y2H assay. f Split luciferase assay showing the interaction of VdNat1 and VdArd1 in N. benthamiana leaves. Photographs were taken 48 h post infiltration. g Immunoblotting showing the overall protein lysine acetylation levels in wild-type V592, VdΔnat1 and VdΔard1 mutant. Ponceau staining showing the loading controls of total proteins. The experiment was repeated three times and showed similar results. h The sensitivity of the VdArd1 knockout mutant and overexpression strains to TG1-2 culture filtrate. i The growth inhibition rate of VdΔard1 and VdArd1OE was calculated after 5 days of incubation. Data are the means ± SD (n = 3 biological independent repeats). Different letters above the bars indicate significant difference as determined by multiple comparisons based on one-way ANOVA (one-sided) followed by Scheffe correction at P < 0.05. P = 1.46 × 10−4, =3.67 × 10−4, =3.73 × 10−6 in sequence. j Immunoblotting showing the overall protein lysine acetylation levels in wild-type V592 before and after treated with TG1-2 culture filtrate (left panel) and 200 μΜ surfactin (right panel) at 48 h. The experiment was repeated three times and showed similar results. k Relative mRNA level of VdNat1 and VdArd1 in wild-type V592 treated with TG1-2cf at different treatment time. The relative mRNA level was examined by quantitative real-time PCR. VdNat1-specific and VdArd1-specific primers were used to amplify VdNat1 and VdArd1, and the Elf (Verticillium elongation factor1-α) was used as an internal control. Data are the means ± SD (n = 3 biological independent repeats). Different letters above the bars indicate significant difference as determined by multiple comparisons based on one-way ANOVA (one-sided) followed by Scheffe correction at P < 0.05, P = 1.06 × 10−9, =4.62 × 10−9, =1.48 × 10−8, =1.32 × 10−8, =0.0046, = 0.0061 in sequence of VdNat1, P = 1.18 × 10−6, =4.51 × 10−6, =5.93 × 10−6, =1.74 × 10−6 in sequence of VdArd1. l Immunoblotting assay showing the expression of VdNat1-Flag and VdArd1-Flag protein in the transgenic strains treated with TG1-2cf at different treatment time. The experiment was repeated three times and showed similar results. Source data are provided as a Source Data file.

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