Fig. 1: Overall cryo-EM structure of an antiparallel double filament of KpFtsZ in complex with KpZapA tetramer.
From: Structural basis for the interaction between the bacterial cell division proteins FtsZ and ZapA
a Typical raw micrograph of the ZapA-FtsZ complex supplemented with 1 mM GMPCPP. The scale bar represents 20 nm. Similarly, 6070 micrographs were collected (Supplementary Fig. 1). b 2D class averages selected before ab initio reconstruction. c Final sharpened map at an overall resolution of 2.73 Å. The colored region corresponds to the region we reconstructed the model, and each color represents different chains. d The reconstructed model containing six FtsZ monomers and two ZapA tetramers. The coloring is the same as in (c). The directions of two antiparallel FtsZ protofilaments are shown in the right panel.
