Fig. 2: Structure of the EC-DSIF-SPT6-U1 snRNP complex.
From: Structure of a transcribing Pol II-DSIF-SPT6-U1 snRNP complex
a Nucleic acid scaffold used for cryo-EM analysis of the EC-DSIF-SPT6-U1 snRNP complex, with template DNA in dark blue, non-template DNA in cyan and RNA in red. mG: 7-methyl guanosine, bp base pair. b Assembled EC-DSIF-SPT6-U1 snRNP complex on a 4–12% NuPAGE Bis-Tris gel, run in MOPS, stained with Instant Blue. The complex assembly experiment was repeated in triplicate. Source data are provided as a Source Data file. c Structure of the EC-DSIF-SPT6-U1 snRNP complex in top view. Pol II is shown in light grey surface representation except for RPB2 in yellow and RPB12 in lime. Elongation factors SPT6 (blue) and DSIF (green) as well as U1 snRNP are shown in cartoon representation. The backbone of U1 snRNA is colored in lavender, U1-70K in dark purple, U1A in violet and Sm proteins in light pink. Template DNA is colored dark blue, non-template DNA in cyan and RNA in red. SL stem loop.
