Fig. 3: SPT6 interacts directly with U1 snRNP.
From: Structure of a transcribing Pol II-DSIF-SPT6-U1 snRNP complex
a Domain organization of SPT6 and truncated SPT6 constructs. A summary of U1 snRNP and U1A binding to all SPT6 constructs in pulldown assays is shown on the right, together with the dissociation constant (Kd) and number of binding sites (N) determined by ITC for the SPT6-U1A interaction. b, c Pulldown of U1 snRNP using different MBP-tagged SPT6 constructs. The prey protein U1-70K as a part of U1 snRNP is highlighted in a purple oval rectangle. MBP is used as a negative control. IN input, E elution, pSPT6 phosphorylated SPT6. d Pulldown of U1-specific proteins using MBP-tagged SPT6CTR. The prey proteins U1A, U1C, and U1-70KRRM are highlighted in oval rectangles. e Pulldown of U1A using different MBP-tagged SPT6 constructs. The prey protein U1A is highlighted in a violet oval rectangle. All pulldowns were repeated in triplicate. Source data are provided as a Source Data file.
