Fig. 2: Ciliopathy-associated alleles of RSG1 elicit distinct effects.
From: The human ciliopathy protein RSG1 links the CPLANE complex to transition zone architecture
A En face in vivo imaging of a Xenopus multiciliated cell with axonemes labelled by membrane-RFP (blue) and basal bodies labeled with centrin-BFP (green). B–E En face images of single MCCs showing Rsg1 basal body localization for control, and S72P (=human A76P), G114E (=human G118E), and D184W (=human R188W) variants. (b’–e’) merged channels showing Rsg1(green) with centrin (magenta), scale bar = 5 μm. F Graph showing mean ± standard deviation of normalized GFP-Rsg1 basal body fluorescence (see “Methods”). N > 25 cells in 5 embryos across 3 experiments for all conditions. N values and statistic can be found in Supplementary Dataset 3.
