Fig. 2: Cryo-EM reconstructions of cognate and doublet decoding tRNASer3 in the E. coli ribosome.
From: Doublet decoding of tRNASer3 demonstrates plasticity of ribosomal decoding center
a Structure of the decoding center (16S rRNA in yellow, 23S rRNA in blue) with cognate tRNASer3 (dark pink) and mRNA-AGC (light gray). Cryo-EM reconstruction of the ribosomal complex (30S subunit shown in yellow, 50S subunit shown in blue) is shown as inset. b Structure of the decoding center (colored as in a) with doublet-decoding tRNASer3 (dark pink) and mRNA-GCA (green). Double-headed arrow indicates widening of the anticodon loop. Inset shows the A1493-U36 Hoogsteen base pair in the cryo-EM map (mesh). c Superposition and schematic of cognate mRNA-GAC and doublet decoding mRNA-GCA from the two structures showing displacement of G19 by A1493 in doublet decoding (colors as in (a-b)) with cognate anticodon in pale pink). d-e Codon-anticodon stacks in the cognate and doublet-decoding structures (colors as in (a-b)).
