Fig. 4: Live imaging defines cellular events during pLL morphogenesis.
From: Axon targeting of transcriptionally distinct pioneer neurons is regulated by retinoic acid signaling
A–D Stills from a timeseries (Supplementary Movie 4) visualizing formation of the pLL system between 15 and 23 hpf. The pLL placode is marked by the cldnB:memgfp transgene, while pLL progenitors undergoing neurogenesis are visualized by Tg(neurod1:Zebrabow). The pLL placode, otic placode, and the neural tube are outlined by dashed lines. A At the onset of pLL neurogenesis (15 hpf), neural and sensory progenitors are intermixed within the pLL placode. B Neural and sensory progenitors are in a process of separating form the pLL ganglion and primordium, respectively. C Neural and sensory progenitors separate at 18 hpf, while neurites (arrowheads) from pLL progenitors project toward sensory progenitors. D At 22 hpf, pioneer axons (marked by arrowheads in the inset) extend with the migrating pLLP. Scale bar = 10 μm. All images scaled the same.
