Fig. 4: Phage lysis increases the persistence of slower-growing AR cells in the face of spontaneously generated AS cells. | Nature Communications

Fig. 4: Phage lysis increases the persistence of slower-growing AR cells in the face of spontaneously generated AS cells.

From: Phage-mediated peripheral kill-the-winner facilitates the maintenance of costly antibiotic resistance

Fig. 4

a Representative CLSM images of strain ARP,Chl (magenta) in the absence (upper image) or presence (lower image) of phage T6. Non-fluorescent regions are composed of ARP,Chl cells that spontaneously lost plasmid pEF001 and became AS cells. We took the images after 10 days of incubation at 21 °C in the absence of antibiotic pressure. b The proportions of the total biomass area occupied by strain ARP,Chl in the absence or presence of phage T6. c The biomass diameters in the absence or presence of phage T6. d The proportions of ARP,Chl cells as a function of the radial distance from the centroid of the biomass. For b–d, each data point is an independent experimental replicate (n = 5), the black data points are for experiments in the absence of phage T6, and the green data points are for experiments in the presence of phage T6. For b, c, the p values are for two-sample two-sided Welch tests. Source data are provided as a Source Data file.

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