Fig. 8: IHC stain of motor neurons in spinal cords from Jun6504-treated vs control (corn oil) treated mice collected at 8 days post-infection.
From: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis
a IHC stain of a transverse section through the lumbar spinal cord of Jun6504-treated animal with no paralysis in hindlimbs. Four panels to the right show increased zoom of the white dashed box where neuronal nuclei, choline acetyltransferase, and DAPI are green, red, and blue signals, respectively. b IHC stain of transverse section through lumbar spinal cord of corn oil control-treated animal with complete paralysis in both hindlimbs. Four panels to the right show increased zoom of the white dashed box where neuronal nuclei, choline acetyltransferase, and DAPI are green, red, and blue signals, respectively. c IHC stain of transverse section through cervical spinal cord of corn oil control-treated animal with complete paralysis in both hindlimbs, but no paralysis in forelimbs. Four panels to the right show an increased zoom of the white dashed box. Anti-neuronal nuclei is green, anti-Enterovirus D68 viral protein 2 is red, and DAPI is blue. d IHC stain of transverse section through lumbar spinal cord of Jun6504- treated animal with complete paralysis in left hindlimb, but no paralysis in the right hindlimb. Four panels to the right show an increased zoom of the white dashed box. Anti-neuronal nuclei is green, anti-Enterovirus D68 viral protein 2 is red, and DAPI is blue. Scale bars in all images are 100 microns in length. Five serial sections (separated by 200 µm intervals) were collected for each spinal cord. A single representative image is shown in Fig. 8, but N = 5 spinal cords were stained for ChAT and N = 3 were stained for VP2.
